STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODY II
نویسندگان
چکیده
منابع مشابه
Staining bacterial smears with fluorescent antibody. III. Antigenic analysis of Salmonella typhosa by means of fluorescent antibody and agglutination reactions.
Moody et al. (1956) and Thomason et al. (1956) have applied fluorescent antibody techniques to the specific detection of Malleomyces pseudomallei in dried smears. This method already has proved successful for the detection and identification of several other pathogenic bacteria. It is potentially a valuable tool for the identification of pathogenic bacteria in clinical specimens and should be o...
متن کاملStaining bacterial smears with fluorescent antibody. II. Rapid detection of varying numbers of Malleomyces pseudomallei in contaminated materials and infected animals.
The identification of pure cultures of Malleomyces pseudomallei in dried smears by use of fluorescent antibody has been described in the previous paper (Moody et al., 1956). The present report describes the application of the method in the detection of these organisms in the presence of other bacteria, in soil, and in animal tissues. The results obtained in this investigation illustrate the pot...
متن کاملRapid fluorescent-antibody staining technique.
The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....
متن کاملStudies on Fluorescent Antibody Staining
1. A study has been made of the non-specific fluorescent staining of splenic imprints treated with fluorescent sheep antibody globulins. 2. In tissue imprints made with the spleens of antigen-stimulated animals, no morphological distinction was evident between areas showing non-specific fluorescence and specific fluorescence. 3. Elimination of non-specific fluorescence was not achieved by any o...
متن کاملDemonstration of Actinomyces and Arachnia species in cervicovaginal smears by direct staining with species-specific fluorescent-antibody conjugate.
For direct observation of microaerophilic actinomycetes by fluorescent antibody, a procedure was developed in which pepsin treatment and rhodamine conjugate of normal serum were used to reduce nonspecific staining in cervicovaginal smears. Actinomyces israelii, Actinomyces naeslundii, and Arachnia propionica were observed in cervicovaginal smears from women who did use and who did not use an in...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1956
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.72.3.362-367.1956